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KMID : 1023420090090040427
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Journal of Dental Hygiene Science
2009 Volume.9 No. 4 p.427 ~ p.433
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The Effect of Over-expression and Inactivation of Nuclear Factor I-C on the Dentin Matrix Gene Expression of MDPC-23 Odontoblasts
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Bae Hyun-Sook
Cho Young-Sik
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Abstract
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Nuclear factor I-C (NFI-C) null mice demonstrated aberrant odontoblast differentiation and abnormal dentin formation. In order to elucidate the mechanisms responsible for these changes, we evaluated the expression of dentin matrix gene after over-expression and inactivation of NFI-C in MDPC-23 cells by reverse transcriptation-polymerase chain reaction (RT-PCR) analysis. Collagen type I (Col I), osteocalcin (OC), and dentin sialophosphoprotein (DSPP) expression was decreased after inactivation of NFI-C. However, bone sialoprotein (BSP) expression was dramatically increased after inactivation of NFI-C. ALP and DMP4 expression was not changed after inactivation of NFI-C. The expression of alkaline phosphatase (ALP) and dentin matrix protein 4 (DMP4) was increased after over-expression of NFI-C, while Col I, OC, DSPP, and BSP expression was decreased. These findings suggest that odontoblasts after loss of NFI-C lost the phenotype of odontoblasts and acquired those of osteoblasts.
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KEYWORD
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NFI-C, Odontoblast, Over-expression, Inactivation, Gene expression
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